Cyclic adenosine monophosphate-stimulated transport of amino acids in kidney cortex.

1. Cyclic adenosine 3’,5’-monophosphate and N6-2’-Odibutyryl cyclic adenosine 3’,5’-monophosphate stimulate the active cellular entry and cumulative uptake of several amino acids in rat kidney cortex slices. 2. Parathyroid hormone, known to augment cellular production of cyclic adenosine 3’,5’-monophosphate by stimulating renal cortical adenyl cyclase, increases the renal cortical uptake of amino acids. Antidiuretic hormone and insulin, hormones which do not stimulate renal cortical adenyl cyclase, have no effect on the renal cortical uptake of amino acids. 3. Characteristics of the NG-2’-0-dibutyryl cyclic adenosine 3’,5’-monophosphate stimulation of amino acid transport include a delay in stimulation of transport, blockade of stimulation by inhibitors of protein synthesis, and inhibition of the expression of the effect by either sodium-free incubation or ouabain exposure.